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For quantitative analyses, ratio of cells positive for nuclear -catenin staining to total number of cells was counted as pixel intensity ratio positive for nuclear localization using Image J (NIH, Bethesda, MD, USA)

A one way or two-way ANOVA, followed by Tukeys multiple comparisons test, was employed for analysis in the SNL and formalin tests

gym, jogging) Hair washing you may want to wash your hair the day before the appointment Skin cleansing in areas injected Massages and extreme heat such as spas The areas injected might feel bruised or tender for a few days and you may also notice your migraine symptoms are temporarily more severe or you may have a migraine attack in the days following the treatment