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To experimentally assess the functional consequences of our nrf2a5 mutated allele, we tested its ability to activate the transcription of an ARE-driven firefly luciferase reporter gene following a previously published protocol (Mukaigasa et al, 2012)

Furthermore, the original co-crystallized ligand inside the active site may affect the conformation of the protein and, as a consequence, the binding mode of other ligands

However, there are limitations to the present study

[DOI] [PMC free article] [PubMed] [Google Scholar] 14.Abrams MB, Nilsson I, Lewandowski SA, Kjell J, Codeluppi S, Olson L, et al

Clinical Cognitive and Energy Benefits Human studies demonstrate PQQ's effectiveness across multiple measures of cellular function