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Brain punches were obtained using a cryostat, and RNA was extracted using the Promega RNA extraction kit

After blockage in bovine serum albumin (5%), cells were immunolabeled with primary antibody specific to HIF-1 (AF7087, Beyotime) overnight at 4, and then subjected to 1-h mixture with FITC-conjugated goat anti-rabbit IgG (ab150080, Abcam)