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At each distance, the plates were placed behind a 5 mm PMMA vertical plate to insure electronic equilibrium

For reducing conditions, samples were diluted 1:1 in 2 Laemmli sample buffer supplemented with 2.5% 2-mercaptethanol

Zhang M, Han W, Xu Y, Li D, Xue Q (2021) Serum miR-128 serves as a potential diagnostic biomarker for Alzheimers disease

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HDACs eliminate the acetyl group from histone proteins which are associated with gene silencing while histone acetyltransferases (HATs) cause acetylation relevant to gene transcription