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As no measured soil concentrations of thiourea are available, a quantitative risk characterization could not be performed

PLoS Medicine, 4 (5), e158

At the same time, it was observed clinically that babies who were mainly fed milk for a long time were prone to low copper anemia, so it was inferred that WD should have more high protein such as drinking milk

Assuming you followed the above advice and used an appropriate binding buffer and your protein isnt huge, then a likely reason for this is that the expression yield is much lower than you expected