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After a final wash step, a tetramethylbenzidine (TMB) peroxidase substrate solution (KPL Inc.) was added to each well and absorbance of the developed color was measured by a colorimetric plate reader (Molecular Device)

The chain reaction of the activated complement components results in formation of a lytic complex and several biologically active peptides of low relative molecular mass (anaphylatoxins)

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Nature 638:787795

energy (ATP) is used to transport substances out of the cell against a concentration gradient in multiple different organs including the brain during this phase (Dean et al., 2001