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In fact, in non cross-over design, the placebo effect will not be mixed up with the effect of the test material

On the subsequent days spheroids were washed in DMEM/F12 medium (Corning, USA) and the medium was changed, as follows: Basal medium: S1 MCDB131 (Corning, USA), 1% Glutamax (Thermo Fisher Scientific, Netherlands) + 1% Pen/Strep (Thermo Fisher Scientific, Netherlands), 2.44 mM glucose (Merck Millipore, USA), 29 mM sodium bicarbonate (Merck Millipore, USA), 2% BSA (Capricorn Scientific, USA), 2 l/100 mL ITS-X (Thermo Fisher Scientific, Netherlands)

Gastric bypass surgery can reduce the stomachs ability to produce intrinsic factor, a protein needed for B12 absorption

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