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It's the gap between aligning a peptide protocol with the biology it was designed to exploit versus forcing it to work against circadian receptor rhythms that have been conserved across 200 million years of mammalian evolution

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( a ) Flowchart of the Tn-seq experiment

Deviation of the observed pathway score from the null distribution was quantified by the fraction of times that the simulated score was more extreme than the observed score

Mol Microbiol 105:413425 Schnupf P, Hofmann J, Norseen J, Glomski IJ, Schwartzstein H, Decatur AL (2006) Regulated translation of Listeriolysin O controls virulence of Listeria monocytogenes