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Transcript levels were quantified using the comparative threshold cycle (Ct) method, and relative gene expression was calculated using the 2 Ct method, as described by Livak and Schmittgen 59

From the supernatant, 0.2 mL was taken and mixed with distilled water and 1.0 mL of invertase solution (10 U mL 1 ), then incubated in a water bath at 50 C for 1 h

It also occurs naturally in various foods, including asparagus, avocado, cucumber, green beans, and spinach.9 A tripeptide is a molecule that comprises three precursor amino acids, which in this case are cysteine, glutamic acid, and glycine

Physiol Plant 170:398414

This leads to reduced soil aeration and water conductivity, which severely affects plant growth and development and results in reduced yields (Zhou et al., 2024a, 2024b