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2.8 QRT-PCR Total RNA and protein were extracted using an RNA/Protein Extraction Kit (Beyotime, R0018M)

The supernatant was aspirated leaving behind the protein disc and an additional 1 mL of cold MeOH was added

7 Therapy target for oxidative stress related SCIRI During I/R injury, a large amount of ROS is generated around the injury site, which can cause excessive oxidative stress, leading to neuronal death and accelerating the development of SCIRI

A study reported that the serum ferritin level may also serve as an early indicator of adipose tissue dysfunction, as it is associated with elevated lipid metabolism markers [55]

Regular exercise may also help improve the absorption of certain vitamins and minerals