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Z., Morsy, M

Jul 2012;57(7):406-13

antioxidants such as vitamin C and E may provide limited help in maintaining glutathione, but the chronic oxidative environment created by radiation exposure ultimately results in the loss of glutathione (24)

Tissue processing and Western Blot Frozen aortae or frozen stimulated cells were dissolved in lysis buffer (120 mmol/L sodium chloride, 50 mmol/L Tris, 20 mmol/L sodium fluoride, 1 mmol/L benzamidine, 1 mmol/L dithiothreitol, 1 mmol/L EDTA, 6 mmol/L EGTA, 15 mmol/L sodium pyrophosphate, 0.8 ug/mL leupeptin, 30 mmol/L p-nitrophenyl phosphate, 0.1 mmol/L phenylmethylsulfonyl fluoride, and 1% NP40) for immunoblotting

26 Cognitive function [including Alzheimers disease (AD)] B12 deficiency is common in the elderly, with a prevalence of 1540%, especially in the elderly with mental disease