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Hence this supplementary approach should not be considered for this purpose until this potential role been further elucidated

Mitochondrial oxidative phosphorylation and the NADPH oxidase (Nox) system constitute the two major enzymatic sources of ROS under hyperglycemic conditions and are central to the oxidative injury driving DR development (60, 61)

Confirming our previous results, each of the mutations R 392 A, F 471 A or Y 473 A, abolished the TRAF6 interaction with LMP1 in HEK293 cells, whereas the K 469 A mutation had no negative effect on the interaction between the two proteins

Understanding these methodological differences helps researchers select appropriate protocols for their experimental goals (Pickart et al., 2015)

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