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Moreover, an abundance of iron and susceptibility to oxidative stress have resulted in the hypothesis that erythrocytes might undergo ferroptosis, an iron-driven ROS-dependent RCD [78]

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The 5 targeting region was digested with Sac II/ Pst I and cloned into Sac II/ Pst I pL0034-digested plasmid

Identification of deleterious missense mutation Six pathogenicity prediction web servers were used to predict the deleterious effect of nsSNPs