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For the RT-PCR of cell culture supernatant of VeroE6/TMPRSS2 cells and lungs of infected hamsters, we extracted total RNA by using Isogen (Nippon Gene) and used One step TB Green PrimeScript PLUS RT-PCR kit (Takara Bio)

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Analytical verification procedures, including chromatographic purity profiling and mass spectrometry identity confirmation, are used to verify peptide identity, purity, and batch consistency