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Seeman P, Chau-Wong M et al (1975)

doi: 10.1542/peds.2011-1106 41 MeltzerD

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It should be noted that, in order to avoid any possible fluorescence from a plastic or glass support during analysis with near-UV excitation, cells were grown on quartz plates that were then placed on the microscope stage in 50-mm thermostated Petri dishes, filled with 5 mL of phosphate buffered saline (PBS)

Improvements in pain intensity and frequency, with a minimum improvement of 50%, indicate successful treatment [7]