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Knockdown of each gene diminished GSH levels, and knockdown of Idh2 and Me1 also increased GSSG levels, thus elevating the GSSG/GSH ratio (Figure 6D)

in contrast, only Cu chelator treatment show potent rescue effect on such excess Cu 2+ -induced cell death, suggesting that cuproptosis is distinct from other forms of cell death

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Emission maximum in the present assay was observed to occur at a wavelength of approximately 485 nm