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For NAD + and NADH assays, 20 L (for NAD + assay) or 30 L (for NADH assay) of the neutralized sample extract was combined with 90 L of assay mix consisting of 50 L of 0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) with 2 mM EDTA (pH 7.5), 20 L of 1.2 mM 2,6-dichlorophenolindophenol, 10 L of 20 mM phenazine methosulfate, and 10 L of 2,500 units mL 1 aldolase dehydrogenase

These compounds promoted apoptosis and autophagy in glioma cells by affecting the activities of SOD and CAT ( Melatonin, a free-radical scavenger, exerts antioxidant effects and protects the BBB under hypoxic conditions

Cardoso BR, Duarte GBS, Reis BZ, Cozzolino SMF

[20] Healthy histamine levels should also be present, as stomach acid is not released without histamine

subhirsutum methanolic extract exhibited important pharmacokinetic properties