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doi: 10.1016/j.coviro.2016.06.001 34 YusufYYoshiiTIyoriMMizukamiHFukumotoSYamamotoDSet al

ROS are likely needed at some basal level for wound healing

In AD, brain regions associated with memory and cognition accumulate amyloid-beta (A) plaques and neurofibrillary tangles (NFTs) formed by hyperphosphorylated Tau protein, leading to dysfunction in cortical and hippocampal neurons (Figure 4A

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Briefly, 3 g of each protein sample was mixed with 15 L of 50 mM ammonium bicarbonate (pH 7.8) (Thermo Fisher Scientific), followed by protein reduction with 2 L of 100 mM dithiothreitol (Thermo Fisher Scientific) for 30 min and protein alkylation with 5 L of 2-iodoacetamide (Thermo Fisher Scientific) for an additional 30 min at room temperature in the dark