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Many women notice: Reduced muscle tone Increased abdominal fat Slower metabolism Reduced exercise recovery Increased fatigue Declining bone density These changes are often blamed entirely on estrogen decline, but IGF-1 appears to be deeply involved

In the following months these deteriorated into problems with balance and motor function, numbness in arms and legs, and even delusions and hallucinations

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The precipitates derived from each supernatant were dissolved in Tris buffer (2 mM EDTA/250 mM Tris-HCl) at pH 8.5, reduced with 0.67 M dithiothreitol, alkylated with 1.4 M iodoacetamide and digested with trypsin

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