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Before the assay, cells were washed and incubated in XF Base Medium supplemented with 10 mM glucose, 2 mM L-glutamine, and 1 mM sodium pyruvate, then equilibrated in a non-CO2 incubator at 37 C for 1 h

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Successful completion of the goals of this project will therefore result in a clinical antidote that could be useful in the case of a terrorist attack involving cyanide