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As anticipated, elevating the fermentation temperature to 30 C led to a substantial increase in the maximum DCW, reaching 146 g/L in the YP-based medium and 80 g/L in the molasses/CSL-based medium, compared to the values obtained at 20 C (Fig

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6.2 Hypoxia In the normal adult intervertebral disc (IVD), oxygen partial pressure gradually decreases from about 10% in the outer annulus fibrosus to less than 1% in the central region, placing the entire disc in a state of continuous hypoxia, which may further decrease during degeneration (Urban, 2002)