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Also it occurs at an activated carbon atom, that is next to sp , sp2 carbons: Oxidative Phase-I involving cytochrome P-450 enzymes: Aromatic hydroxylation: Oxidative Phase-I involving cytochrome P-450 enzymes: Alkene epoxidation: Glutathione conjugation For electrophilic drugs and metabolites: Glutathione conjugation Toxicity of aromatic compounds came from the formation of arene oxide during the metabolism that will be attacked by endogenous nucleophile such as proteins, DNA or RNA