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Recent Independent Replication Al-Dulaimi et al., Biogerontology (2025) Al-Dulaimi, Thomas, Matta, and Roberts conducted an independent in vitro evaluation of Epithalons effects on telomere length in human cell lines

The behavioral testing protocol has been described previously in detail (Wright et al., 1999)

500 lL from each sample was transferred to tubes containing 500 ice-cold acetonitrile with internal standard per incubation sample

To assess the half maximal inhibitory concentration (IC50) of FOXO4-DRI on the serum limitation-induced senescent fibroblast and the non-senescent control fibroblast, the fibroblast viability in the 2% FBS induced senescent fibroblast and 10% FBS cultured non-senescent control fibroblast with increasing concentrations of FOXO4-DRI was detected, and the cell viability curves were showed in Supplementary Fig