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It is absorbed into the pulmonary circulation but does not produce unwanted systemic effects as it has a short half-life, as a result of binding to haemoglobin

Piga et al., 2006)

The cells were lysed on ice for 30 min in IP buffer (50 mM Tris/HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 0.5% NP40, 10% glycerol) with protease inhibitor mixture, and the lysates were cleared by centrifugation