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Briefly, 10-fold diluted serum (100 l for each sample) was added to the wells of an OVA-coated ELISA plate (NuncMaxiSorp flat-bottom, Thermo Fisher Scientific), followed by addition of biotinylated rat anti-mouse IgE (R35-72, 2 g/ml, BD Biosciences), horseradish peroxidase-conjugated avidin (40-fold dilution, BD Biosciences), and NeA-Blue (tetramethylbenzidine substrate

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The active drug blocks pain-producing prostaglandins in the tissues, reducing swelling and easing discomfort in arthritic or overworked joints

Energy tends to respond first, mood and focus follow within a week or so, and the longer-term, structural benefits build quietly over months