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The different immune functions performed by DCs, as well as the transition from an immature to a mature state in response to stimulus signals, are modulated by multiple factors, such as selective gene expression, cytokines stimulation, antigen presentation, nutrient metabolism and so on ( Figure 1 The role of amino acids in protein synthesis is well-defined, and a large number of studies in recent years have demonstrated that amino acid metabolism has a profound effect on various types of immune cells, including bone marrow cells and lymphocytes ( in vitro using serum-free culture medium consistent with the average concentration of the plasma amino acid from a healthy volunteer (HCM) and that from patients with ACM and compared DCs function and glucose-amino acid metabolism in each medium

Hereafter, inserts were washed twice with DMEM-F12 before the cells were added

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doi: 10.1016/j.resp.2024.104286, 26 TangS.YanL.-R.MaZ.-G.JiC

wait a few seconds before withdrawing the needle to ensure full delivery