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The inhibition kinetics module of Sigma Plot 13.0 software was used (Systat Software, Inc., Richmond, CA, USA)

Levels of this antioxidant fall as you age, especially after the age 40. And low levels accelerate the aging process and have been associated with disease and premature death

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Most of the mutants showed increased binding affinity, with S71pAcF and S26pAcF having the most significant improvements at 1.7 nM and 1.6 nM, representing a 9-fold increase compared to the wild type