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Mitochondria, the powerhouse of cells, are predominantly found in skeletal and cardiac muscles, contributing more than 90% of ATP required for cell metabolism [3]

Always use low-binding microcentrifuge tubes and pipette tips (siliconized or polypropylene) when handling dilute solutions below 10 mcg/mL, as surface adsorption can reduce effective concentrations by 20-50% in standard labware

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By using ERK/MAPK activator (TPA) and ferroptosis activator (Era), we show that when combined with TPA, the inhibition of SS on the proliferation, migration, and invasion of BC cells could partially be neutralized, indicating that the ERK2/MAPK signaling pathway may be involved in the ability of SS to regulate BC cells

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