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After being washed with PBS, the stained cells (live-gated on the basis of forward and side scatter profiles) were analyzed on a quantitated by BD Accuri C6 flow cytometry (Becton Dickinson) and data were processed using the BD Accuri CFlow software program (Becton Dickinson)

To overcome this problem, various measures can be taken: (I) selective inhibitors: addition of specific inhibitors can help suppress interfering enzymes while measuring ALP activity

As a result of glutathione deficiency, oxidative stress increases and tissues become vulnerable to damage

The lenses were further incubated for 23 days and photographed under a microscope

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