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These bonds will be cleaved within the acidic environment in endosomes and lysosomes, which will expose the positively charged amino groups of the polymer backbone and trigger the influx of H + and Cl , resulting in the elevation of endosomal osmotic pressure and the inflow of H 2 O

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After resin washing, tryptic digestion, IodoTMT-labeling, and DTT elution 42 , the peptides were dissolved in a final volume of ~25 L water and 20 mM DTT was added to prevent the oxidation of cysteines prior to LC-MS/MS analysis

Often in error, but never in doubt