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CCDC50 promotes tumor growth through regulation of lysosome homeostasis

Three days later, cells were fixed in iced cold 4% of paraformaldehyde at 4C for 20 min, then stained with 50 L of 0.1% crystal violet in 20% methanol on orbital shaker (room temperature, 30 min)

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In 1969, the anticancer effect of CP was evaluated in the sarcoma mouse model (Rosenberg et al., 1969), and by 1971, CP had entered phase I clinical trials and was finally approved by the US Food and Drug Administration for the management of cancers in 1978 ( It is currently well established that CP mediates its tumoricidal effects through binding to DNA, causing formation of intra- and interstrand cross-links