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Microbial metabolism of dietary components to bioactive metabolites: Opportunities for new therapeutic interventions

The knee joints were dissected longitudinally, tissue sections (10 mm) were placed on slides followed hematoxylin and eosin staining

And then, 0.5 L phi29 DNA polymerase, 5 L 10 phi29 DNA polymerase buffer (330 mM Tris-acetate, pH 7.9 at 37 C, 100 mM Mg-acetate, 660 mM K-acetate , 1% (v/v) Tween 20, 10 mM DTT), 2.5 L dNTPs (with a final concentration of 500 M) were introduced to the above mixture (total volume: 50 L)

Muoz K, Blaszkewicz M, Degen GH (2010) Simultaneous analysis of ochratoxin A and its major metabolite ochratoxin alpha in plasma and urine for an advanced biomonitoring of the mycotoxin

What it does is more specific: it stimulates the pituitary gland to produce more of the bodys own growth hormone, which activates lipolysis in visceral adipose tissue, the deep abdominal fat that wraps around organs and drives metabolic risk