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16 (3): p

Each point represents one individual bead (in decreasing order of concentration, WT: n = 5, 9, 6, 9, 12, 3

The following primers to amplify the fragment were designed using the homologous recombination method: GRFT-SmaI(F): 5-GtctcgaggaccggtcccgggATGAAGGCTTTCACTCTTGCCT-3 and GRFT-PmlI(R): 5-ctggtcaccaattcacacgtgATACTGCTCGTAATAGATATCAAGGGA-3

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The musculoskeletal system is a comprehensive and interconnected system, and diabetic patients with muscle wasting syndrome are more prone to developing osteoporosis, and lower muscle mass and strength, along with higher fat content, can impair bone quality (Herrmann et al., 2020)