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Zhang et al., 2004), the affinity and specificity was not as high as expected for the high flux of GSH within plant cells

Although morphologically heterogeneous (Fig

Semin Thorac Cardiovasc Surg 33(4):1158-1168, 2021

Given that isoelectric focusing necessitates the free migration of proteins based on their charge properties within an electric field, lower concentrations of polyacrylamide gels (e.g., 4%) are typically used to prevent molecular sieving

Cells were resuspended in lysis buffer (50 mM sodium phosphate, pH 7.5, 500 mM NaCl, 0.5 mM TCEP, 5% glycerol, 1.0% Triton X-100, 0.1 mM PLP, 2 mg/ml lysozyme) and lysed by sonication