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2003;162:16611667 Gardi C, Arezzini B, Fortino V, Comporti M

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Determination of cell death by PI staining Cells were trypsinized, washed with PBS and suspended in 300 l Dulbeccos phosphate buffered saline (DPBS) supplemented with 0.2% BSA containing 0.3 g/ml propidium iodine (PI, Sigma-Aldrich)

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