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For the glutamine deprivation assay, complete cell-culture was removed, and cells were washed with PBS and then incubated in RPMI lacking glutamine (Vitrocell) supplemented with 10% charcoal-stripped FBS (Invitrogen), for all experiments media was replaced every 48 h

Nutrient deficiencies can not only exacerbate existing anxiety symptoms but also provoke their occurrence in predisposed individuals

On the role of 4-hydroxynonenal in health and disease

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