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Description

The resulting 2.5% SSA plasma supernatant was collected and mixed 1:2.5 with assay buffer to produce a final dilution of 1:5 before assaying

Mixing can cause degradation or altered bioavailability

Danaei, M., Dehghankhold, M., Ataei, S., Davoudi, F

Nucleic Acids Res 53:D1162D1172 Liang X, Hu M, Rogers CQ, Shen Z, You M (2011) Role of SIRT1-FoxO1 signaling in dietary saturated fat-dependent upregulation of liver adiponectin receptor 2 in ethanol-administered mice