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In a preferred embodiment, the detection of the optical purity of L-3-cyano -2 - hydroxypropyl trimethyl ammoniumin and the content of its dextroisomer in step (3) of the present invention includes the following steps: ( 1 ) Preparation of the derivatization reagent solution: D-type or L-type optical pure compound of formula (II) is dissolved in solvent to prepare a 0.01 100 mg / ml solution under the dark conditions