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Description

Redox profiling was done for both plasma and red blood cells using NADMED methodology

The fluorescence was analyzed by flow cytometry (Attune NxT, ThermoFisher Scientific)

Bhat NR, Thirumangalakudi L

Subsequent purification included size exclusion chromatography using a Superdex 200 column (GE Healthcare) and further purification steps with glutathione Sepharose 4B and Ni Sepharose resins to remove residual GST and TEV protease