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Western Blot Total protein content of cell lines or the tumor was extracted by lysis in radioimmunoprecipitation assay buffer containing protease inhibitor cocktail and the protein phosphatase inhibitor, and the total protein concentration was quantified by the BCA (bicinchoninic acid) method

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"It had a section to which it belonged and therefore further exploration of those issues was not required." The World Anti-Doping Authority (WADA) issued a statement on April 22 this year, saying AOD-9604 was a substance still under pre-clinical and clinical development and has not been approved for therapeutic use by any government health authority in the world

This was done in humans, but they did some knockout studies in parallel