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Description

Redza-Dutordoir M, Averill-Bates DA

Results PfGrx(SSG)-dependent oxidation of reduced wild-type and monothiol roGFP2 GSSG rapidly oxidizes roGFP2 in the presence of glutaredoxins in vitro and in vivo 33,34,35,61,62

doi: 10.1046/j.1365-2362.2000.00736.x

To analyze the glutaredoxin-dependent oxidation kinetics for recombinant roGFP2, we first confirmed the fluorescence spectra of reduced and oxidized wild-type roGFP2 (roGFP2 WT (SH) 2 and roGFP2 WT (S 2 ), respectively) using a stopped-flow spectrofluorometer (Fig