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When encephalitogenic T cells collaborate with microglia in multiple sclerosis

Science 2020;368:965

Competition assays for binding of K1B , cK1-1f , or cK1-2f to recombinant MET-Fc protein (Recombinant human HGFR/c-MET-Fc chimera His-tag protein, carrier free, R&D Systems, 358-MT-100/CF) in competition with increasing concentrations of NK1 protein were performed in 384-well microtiter plates (OptiPlate-384, PerkinElmer, CA, USA, 40 L of final reaction volume)