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[] Mercury-induced oxidative stress was associated with decreased activity of the antioxidant enzymes and extensive lipid peroxidation associated with the generation of MDA at high levels

ChemBioChem , 2012 , 13 , 2521-2526 DOI:10.1002/cbic.201200536 89

However, considering the low concentration of O 2 in mitochondria and standard reduction potential of O 2 (0.160 V, pH 7.0), its production will always be favorable even if [O 2 ] were as low as 1 M (Murphy, 2009)

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When transfections were performed in a 12-well plate, these were performed in a similar manner as for 24-well plates, with the exception that the previous day 0.3 10 6 cells were seeded and 4 l of lipofectamine 2000 were used