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KunLiang G., et al., Analytical Biochemistry

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Ageing exacerbates ribosome pausing to disrupt cotranslational proteostasis

In Vitro Maturation, Fertilization and Preimplantation Embryo Culture For in vitro maturation (IVM) of oocytes, every 50 thawed COCs was sequentially cultured in 500 l MI- and MII-medium in four-well culture plates (Nunc, Roskilde, Denmark) at 38.5C in an atmosphere with 5% CO 2 and saturated humidity for 22 h each

Oxidative stress and other stimuli, such as cytokines, activate various signal transduction pathways, leading to the activation of transcription factors, such as NF-kB and AP-1 28