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Cell viability was measured by assaying mitochondrial activity using MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) transformation, as previously described ( Measurement of Intracellular Ca 2+ Concentration Intracellular calcium concentration ([Ca 2+ ] i ) was measured using the fluorescent indicator fura-2

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Additionally, Dolly the sheep was the first animal to ever be successfully cloned from cultured somatic cells 27 , making it possible to use the somatic cell nuclear transfer (SCNT) technique for both biomedical and agricultural applications of sheep 28