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Avoid freezing them, as this can compromise the molecular structure of the compound

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Media was replaced every 2 days and monolayer integrity measured by TEER ( cm 2 , Millicell-ERS Voltohmmeter, Merck Millipore) every 7 days (16 h after media change)

Yang N, Liang B, Srivastava K, Zeng J, Zhan J, Brown L, et al

A phase I study of indoximod, another IDO inhibitor, in combination with docetaxel, an antimitotic chemotherapy drug, showed stable or partially stable disease conditions in more than 50% of patients [132]