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Immunoprecipitation Cells were harvested 36 h post-transfection and then lysed in radioimmunoprecipitation assay (RIPA) lysis buffer (50 mM Tris-HCl, 150 mM NaCl, 0.5% sodium deoxycholate, 1% IGEPAL, 1 mM NaF, 1 mM Na3VO4) containing a protease inhibitor cocktail and a phosphatase inhibitor cocktail (Sigma)

J Pharmacol Exp Ther 261:910917

Usage Ideas The mild taste and tender leaves of spinach makes it extremely versatile either fresh or cooked

This observed transcriptional shift in the SEC population aligns with established histological reports of sinusoidal rarefaction and loss of integrity in the aged marrow 27,52

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