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doi: 10.1016/S0168-8278(22)01680-4 27

The exchange into peptidisc was done by adding 1.5 ml of peptidisc buffer (75 mM NaCl, 20 mM TrisHCl 7.5, 5 mM choline chloride, 0.5 mg/ml peptidisc) to the column, letting the buffer flow through, reapplying the flow-through to the column, and repeating for 20 min