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0.5 ml 0.1%, trichloroacetic acid (TCA), leaf extract supernatant, 0.5 ml of 100 mM potassium phosphate buffer, and 2 ml reagent (1 ml KI w/v double-distilled water) were added in the reaction mixture

doi: 10.7717/peerj.20225, 26

What Happens When Recycling Slows Down In patients, the enzyme GR, which is responsible for recycling spent/oxidised glutathione back to fully loaded, slows down, which leads to the accumulation of oxidised glutathione (GSSG) and the depletion and inability to produce GSH

Immune System Function Your immune cells rely on glutathione to function optimally

This is exactly why glutathione supplements sit under "detox" categories, including this one