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Active Cas9 expression was validated by flow cytometry analysis following infection with a self-cutting mCherry plasmid, which expresses mCherry and an sgRNA targeting the mCherry gene

1 Introduction Kidney disease is a major global non-communicable disease (NCD), with a global prevalence exceeding 10% [affecting ~850 million people when including acute kidney injury (AKI), kidney failure, and dialysis/transplant recipients] ( Cell death is a finely regulated process that occurs through various molecular pathways ( Recent studies have confirmed that the kidney is particularly susceptible to redox imbalance, and ferroptosis plays a significant role in the pathophysiology of various kidney diseases, emerging as a new research hotspot in the field of renal fibrosis ( Figure 1

Regulation of Autophagic Flux by the 20S Proteasome

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